HomePrecision Interference FiltersFilters by ApplicationFluorescence Filters > High Image Quality Fluorescence Microscopy Filter Sets
  • Product: High Image Quality Fluorescence Microscopy Filter Sets
  • Item No.: 6

HB Optical has a long history of innovative design and customizing optical filters for Fluorescence applications. Imaging quality fluorescence filter sets manufactured using IAD technology with better throughput and provide more durable coatings for adverse environmental conditions. They achieve high throughput while maintaining quality  imaging with high-contrast, clear Image quality  and without distortion.

Property

  • High transmission  (T>90%)                                               
  • Excellent out-of-band blocking(Optical Density> OD6)
  • High signal-to-noise ratio
  • High surface accuracy (Transmitted wavefront error: <0.5λ) 
                                                                                                                    
        

 

Filter Set No.

Compatible Fluorophores

Description

AF001

4-Methylumbelliferone7-Amino-4-methylcoumarin

7-Hydroxy-4-methylcoumarin (pH 9),  more

Exciter:365-50nm

Emitter:450-65nm

Dichroic: 405nm

AF002

5-Carboxyfluorescein (5-FAM)Alexa Fluor® 488BODIPY® FL,more

Exciter: 475-40nm

Emitter:535-45nm

Dichroic: 501nm

AF003

5-Carboxynaphthofluorescein

5-ROX (carboxy-X-rhodamine)       

Alexa Fluor® 568

Alexa Fluor® 594 BO-PRO™-3, more

Exciter :560-55nm

Emitter:645-75nm

Dichroic: 599nm

AF004

6-Carboxyrhodamine 6G (6-CR-6G)

6-JOE, Acridine orange (+DNA),      

Alexa Fluor® 532,

 Calcium Green-1™, more

Exciter: 500-25nm

Emitter:545-35nm

Dichroic: 518nm

AF005

5-Carboxytetramethylrhodamine (5-TAMRA), Alexa Fluor® 546, 

Alexa Fluor® 555, BODIPY® 530/550, BODIPY® 558/568, more

Exciter: 525-25nm

Emitter:595-60nm

Dichroic: 559nm

AF006

BO-PRO™-1, Chromomycin A3               

BOBO™-1, Cerulean                      

CFP (Cyan Fluorescent Protein) PO-PRO™-1

Exciter 440-21nm

Emitter:480-30nm

Dichroic: 460nm

AF007

Alexa Fluor® 633, Alexa Fluor® 647            

HiLyte 647 Plus, HiLyte Fluor™ 647        more

 

Exciter: 607-75nm

Emitter:695-55nm

Dichroic: 653nm

 

  •  Customization for special specification and requirement is available. Please contact us to discuss your requirements.

AF001

Optimized for these fluorophores

4-Methylumbelliferone, 7-Amino-4-methylcoumarin, 7-Hydroxy-4-methylcoumarin (pH 9), Alexa Fluor® 350, AMCA/AMCA-X, Calcein Blue, Calcofluor® White, Cascade Blue®, DAPI, Fast Blue, Hoechst 33342 & 33258, LysoSensor™ Blue (pH 5), LysoTracker® Red, Marina Blue®

Sets description

Filter

Part Number

Transmission

Center Wavelength

(nm)

Bandwidth

(nm)

Exciter

AF1001-365-D25

> 40%

365

50

Emitter

AF2001-450-D25

> 85%

450

65

 

 

 

Transmission

Band(nm)

Reflection Band(nm)

Dichroic

AF3001-400-21×29

420-800nm

315-380nm

 

AF002

Optimized for these fluorophores

5-Carboxyfluorescein (5-FAM), Alexa Fluor® 488, BODIPY® FL, Calcein, Cy2®, DiO (DiOC18(3)), EGFP (enhanced),  Evrogen Tag GFP, Evrogen Turbo GFP, Fluo-4, Fluorescein (FITC), Fluoro-Jade, FluorX®, HiLyte Fluor™ 488, MitoTracker® Green, Oregon Green® 488, Rhodamine 110, SpectrumGreen®, SYTO® 13, YO-PRO®-1, YOYO®-1, ZsGreen1

Sets description

Filter

Part Number

Transmission

Center Wavelength

nm

Bandwidth

nm

Exciter

AF1002-475-D25

> 80%

475

40

Emitter

AF20022-535-D25

> 85%

535

45

 

 

Transmission

Band(nm)

Reflection Band(nm)

Dichroic

AF3002-455-21×29

510-700

450 -495

 

AF003

Optimized for these fluorophores

5-Carboxynaphthofluorescein, 5-ROX (carboxy-X-rhodamine),  Alexa Fluor® 568, Alexa Fluor® 594, BO-PRO™-3, BOBO™-3, BODIPY® 581/591, BODIPY® TR-X, Calcium Crimson™, Cy3.5®, HcRed, Lissamine rhodamine B, LysoTracker® Red, MitoTracker® Red, mPlum, mRaspberry, R-phycocyanin, Resorufin, Rhodamine Red™, SpectrumRed®, Texas Red®/Texas Red®-X, X-Rhodamine (XRITC), YO-PRO®-3, YOYO®-3

Sets description

Filter

Part Number

Transmission

Center Wavelength

(nm)

Bandwidth

(nm)

Exciter

AF1003-560-D25

> 85%

560

55

Emitter

AF2003-645-D25

> 85%

645

75

 

 

Transmission

Band(nm)

Reflection Band(nm)

Dichroic

AF3003-595-21×29

610-750

538-585

AF004

Optimized for these fluorophores

6-Carboxyrhodamine 6G (6-CR-6G, 6-JOE, Acridine orange (+DNA), Alexa Fluor® 532, Calcium Green-1™, Cl-NERF (low pH),  DM-NERF (4.5-6.5 pH, Eosin, Erythrosin, Fluo-3, JO-PRO™-1, JOJO™-1, LysoTracker® Green, Magnesium Green™, mCitrine, Oregon Green® 500, Oregon Green® 514, Rhodamine 123, Rhodamine Green™, Sodium Green™, SYTO® 11, SYTOX® Green, TO-PRO®-1, TOTO®-1, YFP (Yellow Fluorescent Protein)

Sets description

Filter

Part Number

Transmission

Center Wavelength

nm

Bandwidth

nm

Exciter

AF1004-500-D25

> 80%

500

25

Emitter

AF2004-545-D25

> 85%

545

35

 

 

Transmission

Band(nm)

Reflection Band(nm)

Dichroic

AF3004-525-21×29

525-700

480-512

 

AF005

Optimized for these fluorophores

5-Carboxytetramethylrhodamine (5-TAMRA),  Alexa Fluor® 546, Alexa Fluor® 555, BODIPY® 530/550, BODIPY® 558/568, BODIPY® 564/570, BODIPY® TMR, Calcium Orange™, CoralHue Kusabira Orange (mKO), Cy3®, DsRed Monomer, Evrogen Turbo RFP, HiLyte 555 Plus, HiLyte Fluor™ 555, Kodak X-Sight 549, MitoTracker® Orange, mTangerine, PKH26, PO-PRO™-3, POPO™-3, Pyronin Y, R-phycoerythrin (PE),  Red Fluorescent Protein (DsRed2), Rhod-2, Rhodamine B, Rhodamine Phalloidin, SpectrumOrange®, SYTOX® Orange, Tetramethylrhodamine (TRITC)

Sets description

Filter

Part Number

Transmission

Center Wavelength

nm

Bandwidth

nm

Exciter

AF1005-525-D25

> 80%

525

45

Emitter

AF2005-595-D25

> 85%

595

60

 

 

Transmission

Band(nm)

Reflection Band(nm)

Dichroic

AF3005-560-21×29

570-750

503-547

 

AF006

Optimized for these fluorophores

BO-PRO™-1, Chromomycin A3, BOBO™-1, Cerulean, CFP (Cyan Fluorescent Protein),  PO-PRO™-1, POPO™-1, SpectrumAqua®, SYTO® 45, SYTOX® Blue

Sets description

Filter

Part Number

Transmission

Center Wavelength

nm

Bandwidth

nm

Exciter

AF1006-440-D25

> 70%

440

21

Emitter

AF2006-480-D25

>85%

480

30

 

 

Transmission

Band(nm)

Reflection Band(nm)

Dichroic

AF3006-455-21×29

465-700

415 - 450

 

AF007

Optimized for these fluorophores

Alexa Fluor® 633, Alexa Fluor® 647, HiLyte 647 Plus, HiLyte Fluor™ 647

Sets description 

Filter

Part Number

Transmission

Center Wavelength

nm

Bandwidth

nm

Exciter

AF1007-607-D25

> 85%

607

75

Emitter

AF2007-695-D25

> 85%

695

55

 

 

Transmission

Band(nm)

Reflection Band(nm)

Dichroic

AF3007-650-21×29

668-900

570-642